Background:
@EN Vibrio vulnificus causes severe wound infection and life-threatening septicemia. In this study we present the characteristics of V. vulnificus bacteriophage and the bases of pathogenicity study of V. vulnificus related on bacteriophage.
@ES Methods:
@EN We isolated bacteriophage for V.vulnificus from marine products and examined its physiological and biochemical characteristics, and the ultrastructure by electron microscopy (TEM, SEM). And also, we investigated host ranges, temperature
stability,
pH stability, inactivation by UV irradiation, DNA isolation and analysis and structural protein analysis of the bacteriophage.
@ES Results:
@EN The adsorption rate of bacteriophage was above 80% and had a little influence at 10-45℃ and pH5-10. Isolated bacteriophage was used to produce antiserum against rabbits and its antigenicity was investigated by cross-neutralization. Western
blot
analysis showed the capsid protein of bacteriophage had antigenicity. The bacteriophage had double-strand DNA. In restriction analysis, the phage DNA was digested Hinf I. The structural protein of phage showed two bands of 34.7kDa and 18.4kDa on
SDS-PAGE.
@ES Conclusion:
@EN The specificity of bacteriophage may be due to the specific binding site that be possessed by host strain surface. Therefore, it seems that virulent bacteriophage controls the number of host strain and then takes a role on natural marine
environment.
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